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Experimental design issues for gene expression microarrays

Kerr, K (Washington)
Tuesday 12 August 2008, 14:30-15:00

Seminar Room 1, Newton Institute


The reference design is a practical and popular choice for microarray studies using two-color platforms. A "reference" RNA is the linchpin of the design, so an important question is what to use as the reference RNA. I will propose a novel method for evaluating reference RNAs and present the results of an experiment that was designed to evaluate three common choices of reference RNA. I will also discuss advantages of reference designs, and issues on the interpretation of the microarray signal.


[ppt ]




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