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Quantitative imaging of migrating cells in vitro and in living tissues

Presented by: 
Christoph Möhl Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)
Date: 
Thursday 23rd July 2015 -
15:15 to 16:30
Venue: 
INI Seminar Room 1
Abstract: 
During the past 20 years, imaging of specific proteins in living biological systems has become one of the most powerful techniques to investigate processes of life on the micro scale. By optical imaging of genetically encoded fluorescent reporters, the localization and dynamics of functional proteins can directly be observed in artificial cell culture systems as well as in whole animals. For studying the process of cell migration specific parts of the cytoskeleton (e.g. actin bundles or substrate adhesion sites) can be recorded in time-lapse movies. However, due to technical limitations it is not feasible to observe multiple target structures simultaneously in one experiment. Thus, the interplay of multiple cellular components such as the contractile actin-myosin network and force-transducing adhesion sites is difficult to investigate. I will discuss opportunities and limitations of optical imaging for studying cell migration by examples from my work on single cell migration as well as collective cell migration during embryonic development. Amongst others, I will present methods how to quantify cellular dynamics with image processing methods and how to integrate heterogeneous data from multiple independent observations to one consistent picture.
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University of Cambridge Research Councils UK
    Clay Mathematics Institute London Mathematical Society NM Rothschild and Sons