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Modeling and tracking glycan diffusion near integrin adhesions using biorthogonal click-chemistry and interference microscopy

Presented by: 
Benjie Ovryn
Wednesday 16th September 2015 - 10:00 to 11:00
INI Seminar Room 1
The dynamics of integrin adhesion to the extracellular matrix (ECM) is critical for cell motility and growth, yet metastatic cells are capable of anchorage-independent survival with loss of adhesion from the primary tumor and subsequent adhesion in the microenvironment of the metastatic niche. Unfortunately, we do not yet have a clear understanding of the detailed mechanisms that govern the nucleation, clustering and adhesion of integrins to the ECM in the presence of the myriad of cell-surface glycoproteins that extend into the extracellular space. In order to explore how interactions between integrins and glycans alter clustering and adhesion, we have adopted a three-pronged approach that includes: (1) modeling the energetic landscape that governs membrane bending and integrin adhesion; (2) tracking bioorthogonally tagged cell-surface glycans with single-molecule sensitivity and (3) measuring membrane topography using phase-shifted laser feedback interference microscopy.
University of Cambridge Research Councils UK
    Clay Mathematics Institute London Mathematical Society NM Rothschild and Sons